Curriculum vitae
Filipe André da Silva Raminhos Natálio
E-mail 1: natalio@uni-mainz.de
E-mail 2: filipenatalio@yahoo.com
Nationality: Portugal
Academic degrees:
Licenciatura/First Class in Chemistry, July 2004, Faculty of Sciences, University of Lisbon, with the final classification of 15 out of a possible 20.
Scientific Experience:
May 2005 until present -
Ph.D. student – Marie Curie Fellowship - 18 months - Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Mainz Universität, Germany
February 2005 until May 2005:
Post-graduated scholarship, FCT (Foundation for Science and Technology). Enzymatic reactions of oxygen-transfer (sulfoxidation) using several organic substrates with pharmacological interest and enzyme from different biological sources. Sulfoxidation mechanistic approach. Enantioselectivity and yield optimization. The project will be developed in collaboration with INETI (Lisbon, Portugal) and Faculty of Medical School (Lisbon, Portugal). Chemistry and Biochemistry Department, Faculty of Sciences, University of Lisbon
January 2004 until June 2004:
Development of project involving microbiology. Extraction, purification and characterization of alkaline phosphatases from Gram-negative bacteria. Kinetic studies. University of Lisbon - Faculty of Sciences.
Design of new enzymes
Enzymatic reactions using biological active organic substrates produced by bacteria – N-acyl homoserine lactones (AHL’s) – Mechanistic approach and interpretation of phosphatases-boron-signalling molecules co-relation on interspecies communication, University of Lisbon - Faculty of Sciences.
October 2002 to March 2003:
Development of several projects within the theme vanadium haloperoxidases: sulfoxidation using enzyme mutants - a mechanist approach to oxygen- transfer enantioselectivity. Kinetic studies (inhibition studies) and design of new boron-containing enzyme. Purification of heme-containing peroxidases, University of Amsterdam – Institute for Molecular Chemistry
October 2001 to July 2002:
Biological chemistry of vanadium: vanadium haloperoxidases - purification, characterization and kinetics studies. University of Lisbon - Faculty of Sciences.
Development of semi-synthetic haloperoxidase, University of Lisbon - Faculty of Sciences and Technical Superior Institute - Technical University of Lisbon
Haloperoxidases in marine sponges - the impact in Marine Environment, University of Lisbon - Faculty of Sciences
October 2000 to September 2001:
Screening of oxidoreductases in marine sponges University of Lisbon - Faculty of Sciences
Participation in congresses: Posters and oral comunications
M. Nicolai, F. Natalio, G. Gonçalves, M. Almeida, and M. Humanes, “Iodoperoxidation of tyrosine derivatives by Vanadium Haloperoxidases”, 12th International Conference on Biological Inorganic Chemistry - ICBIC-12, University of Michigan, USA, July 31- August 5, 2005
A. Silva, F. Natálio, A. Alexandre, M. Almeida, M. Humanes, “Tyrosine derivatives iodination by vanadium haloperoxidases”, 7th FIGIPS, Faculty of Sciences, University of Lisbon, 11-14 June 2003
C. Almeida, F. Natálio, S. Marcão, M. Almeida, A. Conceição, T. Barbosa, M.T.Lopes, M.F. Araújo, M. Humanes, “The presence of haloperoxidases in marine sponges”, Marine Chemistry Symposium, University of Algarve, 2- 4 April 2002
F. Natálio, S. Marcão, M. Almeida, C. Duarte, M.T. Lopes. M. Humanes “Oxido-redutases em Demospongiae da Costa Portuguesa”, Iberic Conference of Environmental Protection, Piaget Institut, 12-14 July 2001
Publications:
2005 – F Natálio, M. Rothenberger, O Boreiko, W. Tremel, A. Reiber, H.-C. Schröder and W.E.G.Müller ”Spicule formation in siliceous sponge Suberites domuncula”, Conference Proceedings, European Marie-Curie Conference, 28th-30th September, Pisa-Livorno
2005 – F. Natálio, M. Almeida, M. Humanes “Review of vanadium haloperoxidases” –Bulletin of Portuguese Chemical Society, July-September 2005
2004 – F. Natálio, A. Alexandre, M. Almeida, C. Duarte, M. Humanes “Tyrosine derivatives iodination by vanadium haloperoxidases” – submitted to Journal Biological Chemistry
2002 – Publication Book chapter, Piaget Editions – “Ecotoxicology and Polutent removal in Iberic Peninsula”
Participation in congresses: Attendance
2005
– Marie Curie European Conference – 28th-30th September – Pisa/Livorno, Italy
2001
– Participation in the workshop: “Oxidative Stress in Biology and Medicine”, Auditorium of Faculty of Medicine of University of Lisbon
Participation in congress: Organization
2002
– Seminars: Seminar cycle to promote the ongoing undergraduate projects in Chemistry and Biochemistry department
2001
– Organization of congress EuroCarb XI, from 2nd to 7th of September, Faculty of Sciences, University of Lisbon
Other complementary education:
2005 - Summer school -
Ruder Boscovic Institut - Institute Center for Marine Research – 23-28th August, Rovinj, Croatia
Computational Biology Unit, 19-22th September, Bergen, Norway
Other interests:
Member of the Portuguese Chemical Society and Swiss Proteomics Society Bioinformatics. PC skills MS Word, Excel, PowerPoint, Access and Macromedia Flash MX, Dreamweaver MX in Windows and Macintosh environments and experience with specific scientific software: HLPC, modeller.